5.  Long-Term Exposure Stability of Sol-Gel Immobilized Lipases in Organic Solvents

Authors: P. Borza, F. Peter, I. Hulka and C. Paul


Abstract:

Stability of lipases from Pseudomonas fluorescens (Amano AK), Burkholderia cepacia (Amano PS) and Candida antarctica B (Cal B), immobilized by sol-gel entrapment and sol-gel entrapment combined with adsorption on Celite 545, was investigated by long-term incubation in seven different solvents. The immobilization has been carried out using a ternary silane precursors system consisted of phenyl-trimethoxysilane, vinyl-trimethoxysilane and tetramethoxysilane, at 1.6/0.4/1 molar ratio. The immobilized biocatalysts were incubated in various organic solvents for 7 days, at 25C. The activity and enantioselectivity were assayed in the acylation reaction of 2-hexanol with vinyl acetate. The activity of the immobilized biocatalysts was not significantly altered by the tested organic solvents, while the enantioselectivity values of all immobilized enzymes were higher compared to the native ones. In the case of Cal B lipases the operational stability was excelent as well, the transesterification activity remaining unchanged during 14 reuse cycles.


Keywords: lipase, sol-gel entrapment, stability, organic solvent, reuse

PDF Version

 
 
Issues

Articles in Press
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2016
Volume 61(75), Issue 2, 2016
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2016
Volume 61(75), Issue 1, 2016
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2015
Volume 60(74), Issue 2, 2015
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2015
Volume 60(74), Issue 1, 2015
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2014
Volume 59(73), Issue 2, 2014
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2014
Volume 59(73), Issue 1, 2014
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2013
Volume 58(72), Issue 2, 2013
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2013
Volume 58(72), Issue 1, 2013
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2012
Volume 57(71), Issue 2, 2012
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2012
Volume 57(71), Issue 1, 2012
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2011
Volume 56(70), Issue 2, 2011
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2011
Volume 56(70), Issue 1, 2011
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2010
Volume 55(69), Issue 2, 2010
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2010
Volume 55(69), Issue 1, 2010
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2009
Volume 54(68), Issue 2, 2009
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2009
Volume 54(68), Issue 1, 2009
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2008
Volume 53(67), Issue 1 - 2, 2008
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2007
Volume 52(66), Issue 1 - 2, 2007
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2006
Volume 51(65), Item 1 - 2, 2006
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2005
Volume 50(64), Item 1 - 2, 2005
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)



   
 
 
  © 2017 Industrial Chemistry and Environmental Engineering Faculty